Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 13;8:266. doi: 10.3389/fimmu.2017.00266

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Fabersani, Abeijon-Mukdsi, Ross, Medina, González and Gauffin-Cano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Leptin receptor (Ob-Rb) expression in RAW 264.7 cells. RAW 264.7 cells after lactic acid bacteria (LAB) stimulation were incubated with a polyclonal rabbit antimouse Ob-Rb Ab and a secondary FITC-conjugated Ab (antirabbit IgG). Ten thousand cells were analyzed per sample at an argon laser flow cytometry scanner. (A) Histograms correspond to the analysis of Ob-Rb expression by non-stimulated (negative control) and LAB-stimulated RAW 264.7 cells. The stimulating strains were CRL431, CRL258, CRL1063, CRL66, CRL72, CRL117, CRL1446 CRL1434, CRL350, CRL352, CRL353, CRL355, CRL575, and CRL576. Lipopolysaccharide (LPS) was used as a positive control. Open black line represents fluorescent labeling of Ob-Rb positive cells of negative control, and gray areas represent other strains stimulus. (B) Percentage of Ob-Rb expression in RAW 264.7 cells. The bars represent the different LAB strains. The results are expressed as media of percentages of cells Ob-Rb (+) ± SD of two experiments. Mean values in the same graphic with different letters (a–d) were significantly different (P < 0.05). (C) Microphotograph representative of the Ob-Rb expression in non-stimulated macrophages (negative control, C-1) and LPS-stimulated macrophages culture (positive control, C-2).

Leptin receptor (Ob-Rb) expression in RAW 264.7 cells. RAW 264.7 cells after lactic acid bacteria (LAB) stimulation were incubated with a polyclonal rabbit antimouse Ob-Rb Ab and a secondary FITC-conjugated Ab (antirabbit IgG). Ten thousand cells were analyzed per sample at an argon laser flow cytometry scanner. (A) Histograms correspond to the analysis of Ob-Rb expression by non-stimulated (negative control) and LAB-stimulated RAW 264.7 cells. The stimulating strains were CRL431, CRL258, CRL1063, CRL66, CRL72, CRL117, CRL1446 CRL1434, CRL350, CRL352, CRL353, CRL355, CRL575, and CRL576. Lipopolysaccharide (LPS) was used as a positive control. Open black line represents fluorescent labeling of Ob-Rb positive cells of negative control, and gray areas represent other strains stimulus. (B) Percentage of Ob-Rb expression in RAW 264.7 cells. The bars represent the different LAB strains. The results are expressed as media of percentages of cells Ob-Rb (+) ± SD of two experiments. Mean values in the same graphic with different letters (a–d) were significantly different (P < 0.05). (C) Microphotograph representative of the Ob-Rb expression in non-stimulated macrophages (negative control, C-1) and LPS-stimulated macrophages culture (positive control, C-2).