Table 4.
mRNA expression of selected subunits of the mitochondrial respiratory chain and genes encoding proteins linked to energy metabolism in MA (n = 12), DLB (n = 9), and rpDLB (n = 4).
| Probes | MA | DLB | rpDLB | MA vs DLB | MA vs rpDLB | DLB vs rpDLB | |
|---|---|---|---|---|---|---|---|
| Mitochondria | |||||||
| Complex I | NDUFA2 | 1.02 ± 0.22 | 1.01 ± 0.09 | 0.94 ± 0.08 | – | – | – |
| NDUFA7 | 1.27 ± 0.58 | 0.89 ± 0.25 | 1.00 ± 0.11 | – | – | – | |
| NDUFA10 | 1.03 ± 0.27 | 1.11 ± 0.26 | 1.19 ± 0.31 | – | – | – | |
| NDUFB3 | 1.02 ± 0.23 | 0.95 ± 0.24 | 0.99 ± 0.20 | – | – | – | |
| NDUFB7 | 1.02 ± 0.23 | 1.19 ± 0.53 | 1.25 ± 0.13 | – | – | – | |
| NDUFB10 | 1.05 ± 0.35 | 0.87 ± 0.30 | 1.19 ± 0.20 | – | – | – | |
| NDUFS7 | 1.09 ± 0.47 | 0.98 ± 0.26 | 0.71 ± 0.14 | – | – | – | |
| NDUFS8 | 1.03 ± 0.25 | 1.19 ± 0.66 | 1.64 ± 0.25 | – | – | – | |
| Complex II | SDHB | 1.05 ± 0.38 | 0.84 ± 0.16 | 0.84 ± 0.16 | – | – | – |
| Complex III | UQCRB | 1.06 ± 0.40 | 1.09 ± 0.44 | 1.00 ± 0.24 | – | – | – |
| UQCR11 | 1.02 ± 0.19 | 1.10 ± 0.26 | 1.28 ± 0.27 | – | – | – | |
| Complex IV | COX7A2L | 1.08 ± 0.41 | 0.93 ± 0.39 | 1.07 ± 0.38 | – | – | – |
| COX7C | 1.03 ± 0.24 | 0.89 ± 0.38 | 0.85 ± 0.24 | – | – | – | |
| Complex V | ATP5D | 1.03 ± 0.24 | 0.98 ± 0.18 | 0.93 ± 0.13 | – | – | – |
| ATP5G2 | 1.07 ± 0.42 | 0.61 ± 0.23 | 0.69 ± 0.02 | *↓ (0.0118) | – | – | |
| ATP5H | 1.02 ± 0.21 | 0.80 ± 0.18 | 0.82 ± 0.05 | *↓ (0.0281) | – | – | |
| ATP5L | 1.03 ± 0.26 | 1.00 ± 0.23 | 1.01 ± 0.06 | – | – | – | |
| ATP5O | 1.02 ± 0.18 | 0.86 ± 0.22 | 0.95 ± 0.14 | – | – | – | |
| Energy metabolism-related molecules | |||||||
| ATP2B3 | 1.03 ± 0.25 | 0.99 ± 0.45 | 1.03 ± 0.33 | – | – | – | |
| ATP2B4 | 1.01 ± 0.18 | 0.97 ± 0.27 | 0.83 ± 0.30 | – | – | – | |
| ATP4A | 1.11 ± 0.51 | 2.15 ± 1.00 | 1.75 ± 1.24 | *↑ (0.0389) | – | – | |
| ATP6VOB | 1.04 ± 0.29 | 0.72 ± 0.25 | 0.54 ± 0.10 | *↓ (<0.05) | **↓ (0.0035) | – | |
| FAM82A2 | 1.02 ± 0.22 | 1.05 ± 0.09 | 1.09 ± 0.09 | – | – | – | |
| SLC6A6 | 1.02 ± 0.19 | 1.37 ± 0.27 | 1.39 ± 0.36 | *↑ (0.0154) | – | – | |
| SLC25A3 | 1.05 ± 0.35 | 1.09 ± 0.43 | 1.01 ± 0.19 | – | – | – | |
| TOMM40 | 1.05 ± 0.38 | 0.88 ± 0.30 | 0.56 ± 0.15 | – | *↓ (0.0486) | – | |
| ZNF642 | 1.02 ± 0.22 | 0.94 ± 0.32 | 1.03 ± 0.04 | – | – | – | |
Similar trends are found in DLB and rpDLB when compared with MA cases. Major changes are represented with the corresponding p-value, and mRNA levels are expressed as mean fold change ± SD determined by real-time quantitative PCR (RT-qPCR) and analyzed with the ΔΔthreshold cycle (CT) method: *p < 0.05, **p < 0.01, and ***p < 0.001.