Table 6.
mRNA expression levels of genes encoding nucleolar proteins, ribosomal proteins, and 18S and 28S rRNAs in MA (n = 12), DLB (n = 9), and rpDLB (n = 4).
| Probes | MA | DLB | rpDLB | MA vs DLB | MA vs rpDLB | DLB vs rpDLB | |
|---|---|---|---|---|---|---|---|
| Nucleolar proteins | |||||||
| NPM1 | 1.07 ± 0.45 | 0.60 ± 0.11 | 0.71 ± 0.24 | *↓ (0.0246) | – | – | |
| NCL | 1.075 ± 0.446 | 0.70 ± 0.17 | 0.78 ± 0.27 | – | – | – | |
| UBTF | 1.07 ± 0.43 | 0.72 ± 0.17 | 0.83 ± 0.40 | – | – | – | |
| rRNA | |||||||
| rRNA18S | 1.04 ± 0.28 | 1.22 ± 0.41 | 2.12 ± 0.66 | – | **↑ (0.0015) | **↑ (<0.01) | |
| rRNA28S | 1.12 ± 0.62 | 1.01 ± 0.25 | 1.34 ± 1.12 | – | – | – | |
| Ribosomal proteins | |||||||
| Large subunit | RPL5 | 1.10 ± 0.14 | 0.99 ± 0.16 | 1.23 ± 0.25 | – | – | – |
| RPL7 | 1.01 ± 0.12 | 1.19 ± 0.15 | 1.38 ± 0.20 | *↑ (<0.05) | **↑ (0.0014) | – | |
| RPL21 | 1.03 ± 0.29 | 2.10 ± 0.65 | 2.72 ± 0.77 | **↑ (<0.01) | ***↑ (<0.0001) | – | |
| RPL22 | 1.01 ± 0.17 | 0.78 ± 0.18 | 0.83 ± 0.20 | *↓ (0.0305) | – | – | |
| RPL23A | 1.04 ± 0.31 | 1.41 ± 0.28 | 1.71 ± 0.46 | – | *↑ (<0.05) | – | |
| RPL26 | 1.02 ± 0.21 | 0.76 ± 0.34 | 0.71 ± 0.22 | – | – | – | |
| RPL27 | 1.01 ± 0.15 | 1.04 ± 0.11 | 1.23 ± 0.34 | – | – | – | |
| RPL30 | 1.03 ± 0.26 | 1.55 ± 0.34 | 2.05 ± 0.31 | **↑ (<0.01) | *** ↑ (<0.0001) | – | |
| RPL31 | 1.01 ± 0.15 | 1.21 ± 0.25 | 1.55 ± 0.29 | – | **↑ (0.0026) | – | |
| Small subunit | RPS3A | 1.03 ± 0.25 | 1.36 ± 0.40 | 1.26 ± 0.22 | – | – | – |
| RPS5 | 1.01 ± 0.11 | 1.20 ± 0.17 | 1.31 ± 0.19 | *↑ (<0.05) | *↑ (<0.05) | – | |
| RPS6 | 1.01 ± 0.17 | 1.14 ± 0.10 | 1.70 ± 0.40 | – | ***↑ (0.0002) | **↑ (<0.01) | |
| RPS10 | 1.02 ± 0.23 | 1.04 ± 0.19 | 1.17 ± 0.30 | – | – | – | |
| RPS13 | 1.01 ± 0.16 | 1.04 ± 0.11 | 1.35 ± 0.30 | – | *↑ (<0.05) | *↑ (<0.05) | |
| RPS16 | 1.04 ± 0.29 | 0.75 ± 0.29 | 0.52 ± 0.01 | – | *↓ (0.0166) | – | |
| RPS17 | 1.01 ± 0.11 | 0.90 ± 0.06 | 1.10 ± 0.28 | – | – | – | |
| RPS20 | 1.01 ± 0.13 | 0.92 ± 0.14 | 0.96 ± 0.20 | – | – | – | |
Changes are represented with the corresponding p-value and mRNA levels are expressed as mean fold change ± SD determined by RT-qPCR and analyzed with the ΔΔCT method: *p < 0.05, **p < 0.01, and ***p < 0.001.