Figure 3.

FAD2-1A mutations of the screened four high oleic acid mutants. (A) FAD2-1A gene model in the wild type Forrest. (B) Predicted FAD2-1A protein showing amino acid differences between the Forrest-WT and the four high oleic mutants identified by forward genetics, three missense mutations (R22G, L101F, and P284L), and one silent mutation (L249=) within the FAD2-1A. The oleic acid level represents the highest content obtained from each line. The primers used for TILLING and gDNA sequencing are indicated by arrows.