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FAD2-1B mutations of the screened high oleic acid mutant. (A) FAD2-1B gene model in wild type Forrest. (B) Predicted FAD2-1B protein showing amino acid differences between the Forrest-WT and the high oleic mutant identified by forward genetics; one missense mutations (P163S) within the FAD2-1B. The oleic acid level represents the highest content obtained. The primers used for PCR genotyping are indicated by arrows.
