Figure 3. RPL26 is necessary for proper assembly of polysomes on TAp73 mRNA and mRNA translation.

A. Sucrose density gradient was used to separate polysomes from p53^−/− HCT116 cells transfected with scramble siRNA or siRNA against RPL26 for 72 h. The level of TAp73 and actin transcripts was measured in each fraction. B. The experiment was performed as in A. except that MDM2-knockout p53^−/− HCT116 cells were used. C. MDM2-knockout p53^−/− HCT116 cells were transfected with scrambled siRNA or siRNA targeting RPL26 (siRPL26) for 3 days and the level of newly synthesized TAp73 protein was measured by ³⁵S-metabolic labeling.