Figure 2. AZD reduces protein aggregation and associated toxicity in the cell.

A. Effect on ataxin130Q-GFP aggregation by AZD, or celastrol, (Cel) or DMSO as indicated in Neuro-2a cells. Scale bar 10 μm. B. The aggregates were counted and plotted after 48 h treatment, 450 cells were counted for each treatment, ***p < 0.001. C. Ethidium bromide stained agarose gel showing relative levels of HSP70 (mHSP70) transcripts isolated from cells subjected to identical treatments in duplicate as represented in panel (A) estimated by semiquantitative RT-PCR assays. D. Comparison of sensitivity of Neuro-2a cells to AZD or celastrol after 24 h treatment estimated by MTT assay. Data are mean±SEM, n ≥ 3