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. 2016 Oct 5;7(48):78577–78590. doi: 10.18632/oncotarget.12480

Figure 1. Characteristics of primary epithelial cells derived from patient tissue specimens.

Figure 1

(A) A representative culture of primary cells from a digested tumor biopsy at day 1 (a) and day 5 (b) of culture, respectively. (B) Flow cytometric analysis of luminal (EpCAM+, Claudin4+) and basal (CD49f+, CD10+) markers on four representative tumor primary cells harvested at the time of CELx HSP test. (C) Plot showing the Mean Fluorescence Channel (MFC) of the luminal marker EpCAM (x-axis) and the basal/progenitor biomarker CD49f (y-axis) for all 34 tumor samples tested (filled circles). For comparison, 2 healthy samples are displayed (empty circles). (D) Comparison of expression levels of HER2, ERα, and PR between primary cells and cell lines (HER2+ and HER2−), which were measured by flow cytometry. (E) Histogram plot of HER2 expression measured by flow cytometry of a representative tumor (shaded peak) compared to HER2+ cell line SKBR3 (solid line) and HER2- cell line MDA231 (dashed line) that is coincidentally in the same range as the healthy samples. The bar below the graph represents the range of means from all the primary tumors and healthy tissue tested (MFC range 31-210).