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. 2016 Oct 5;7(48):78577–78590. doi: 10.18632/oncotarget.12480

Figure 2. Optimization and specificity of CELx HSP test in primary cells.

Figure 2

(A) R37 primary cells seeded at different densities (8000, 10000, 12000, 15000 cells per well) in a sensor plate were stimulated with NRG1b (3 nM). CELx curves are displayed using Delta CI values to demonstrate the relative signals normalized to the time point (arrow) when NRG1b was added. Positive correlation between cell number and NRG1-driven CELx signal is shown in the inset. (B and C) Dose-response curves of NRG1b and EGF stimulation of CELx signals in R39 primary cells. (D) Dose-response curve of pertuzumab showing its specific inhibitory effect on NRG1b-driven CELx signal.