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. 2016 Oct 8;7(48):78619–78630. doi: 10.18632/oncotarget.12524

Figure 1. Comparison of ANO1 expression levels in multiple epithelial cell lines.

Figure 1

(A) ANO1 mRNA expression in seven cell lines was determined by quantitative real-time PCR. The mRNA expression of ANO1 was much higher in human keratinocyte HaCaT, prostate cancer cell line PC-3, colon cancer cell lines SW480, HCT116 and HT-29 than that in normal breast epithelium MCF 10A and bronchial epithelial cell line BEAS-2B. (B) ANO1 protein expression in the cell lines was detected by Western blot. Higher expression of ANO1 was confirmed in cell lines of HaCaT, PC-3, SW480, HCT116 and HT-29 than MCF 10A and BEAS-2B. HEK293 cells transfected with ANO1 plasmid were used as positive control. (C) Quantitative analysis of ANO1 protein expression in cell lines from B.