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. 2016 Oct 8;7(48):78619–78630. doi: 10.18632/oncotarget.12524

Figure 2. Silencing ANO1 inhibits cell proliferation.

Figure 2

(A–E) Cell proliferation of the indicated cell lines was assessed by CCK8 assay. Three days after lentiviral infection of ANO1 shRNAs, cells were re-plated into 96-well plates and cell proliferation was measured every day for six days. Silencing ANO1 resulted in significant inhibition of cell viability in a time-dependent manner. Data are expressed as mean ± SEM; n = 4; #P < 0.05, ##P < 0.01 and ###P < 0.001 are for statistical comparisons of ANO1 shRNA 1 versus control shRNA; *P < 0.05, ** P < 0.01 and *** P < 0.001 are for statistical comparisons of ANO1 shRNA 3 versus control shRNA. (F) Representative images of colony formation in soft agar assay. Knockdown of ANO1 by ANO1 shRNA1 or shRNA3 decreased the colony-formation capacity of HCT116 and HT-29 as compared with control shRNA. (G) Quantitative analysis of colony numbers from F. Data are expressed as mean ± SEM; n = 3; ***P < 0.001.