Figure 3. CD133⁺ colon cancer cells and 5-FU sensitivity.

CD133⁺ and CD133⁻ cells were sorted from LS411N A. and SW620 B. cells, respectively. The cells were stained with CD133- and CD44-specific antibodies and analyzed by flow cytometry (top panel). The two subsets of cells were cultured in the presence of 5-FU at the indicated concentrations for 3 days and analyzed for viability by MTT assays.