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. 2016 Oct 4;7(48):78883–78895. doi: 10.18632/oncotarget.12428

Figure 1. SINE compound resistant cell lines showed increased basal levels of NF-κB transcriptional activity.

Figure 1

SINE compound resistant HT-1080 (fibrosarcoma, HT-1080-R) cells were selected by continued exposure of sensitive parental cells in increasing concentrations of the SINE compound KPT-185. A, B. Parental and C, D. HT-1080-R cells were treated with 1μM of selinexor for 4 hours. SINE compound-induced nuclear retention of IκB-α was evaluated by immunofluorescence microscopy and shown to be impaired in SINE compound resistant cells. E. Parental-sensitive HT-1080, HT-1080-R, and ASPS-KY (Alveolar Soft Part Sarcoma) cells were tested for NF-κB transcriptional activity by ELISA assay. Equal number of cells from the 3 cell lines were lysed with RIPA buffer. The results from 2 independent assays show that higher NF-κB transcriptional activity is correlated with lower sensitivity to the cytotoxic effects of selinexor. The error bars indicate the standard deviation and the Student's t-test was used to calculate p values. **p<0.01.