Figure 2. Reduction in the levels of IκB-α affects the potency of selinexor.

A. U-2 OS cells were transfected with either 40nM IκB-α or control siRNA using lipofectamine RNAiMax. At 96 hours post transfection there was a 90% reduction in the protein levels of IκB-α. B. The siRNA transfected and non-transfected U-2 OS cells were treated with increasing concentrations of selinexor and cells were assayed for the cytotoxic effect of selinexor 72 hours post treatment.