Figure 4. In vivo combination of bortezomib with selinexor is more efficacious than either single agent in the SINE compound resistant tumors.

HT-1080-R cells (1×10⁶) were subcutaneously injected in the flank of 3 naïve mice. Four weeks post injection, palpable tumors were formed at which time mice were euthanized, and tumors harvested and 50 mg pieces were re-implanted unilaterally into the flank of experimental groups. Bortezomib (Bortz) at 1mg/kg iv; selinexor (Sel) 15mg/kg orally or the combination of bortezomib at 1mg/kg + selinexor at 15mg/kg was administered Monday and Thursday for 2 weeks. Effects on tumor weight were recorded as described in materials and methods. Data shown are the mean for each group. The bortezomib by itself showed no significant tumor reduction. Selinexor as single agent and in combination showed 50% and 76% tumor weight reduction respectively at the end of the study (day 32). The error bars indicate SEM and the Student's t-test was used to calculate p values. *p<0.05, **p<0.01.