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. 2016 Oct 26;7(48):78918–78931. doi: 10.18632/oncotarget.12912

Figure 6. DDR2 expression is associated with tumorigenic potential by enhancing proliferative, migratory, and invasive ability of tumor cells.

Figure 6

(A) Endogenous DDR2 transcript expression are determined by quantitative RT-PCR. Compared with grade 1 UC cell line RT4, there are two grade 3 UC cell lines showing high DDR2 mRNA expression, including UMUC3 and BFTC909. (B) Both the high DDR2-expressing cell lines were subsequently infected by Lentivirus caring shRNA targeting DDR2 (shDDR2#1 and shDDR2#2). Successful knockdown of DDR2 was confirmed by quantitative RT-PCR. (C) Using 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay to examined cell viability, we demonstrate positive effects of DDR2 expression on cell proliferation. Similar results are identified for cell migratory (D) and invasive ability (E). The quantified results are illustrated as means ± sd. Error bars show the standard error. Data represents mean values of three independent experiments. Student's t-test is used, (*P < 0.05).