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. 2016 Oct 26;7(48):78946–78957. doi: 10.18632/oncotarget.12935

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Copyright: © 2016 Cheng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) Profiles of S100A4 WT and mutants for size-exclusion chromatography on HiLoad^TM 26/60 Superdex^TM 75 prep grade column. (B) SDS-PAGE of purified S100A4 proteins from size-exclusion chromatography. Molecular weight markers (M). (C) Circular dichroism measurements were determined in the amide band (190–240 nm) at 3 mg/ml protein concentration in S100A4 WT and mutants FPLC buffer using a Jasco-715 spectropolarimeter. The raw data were further analyzed by K2D3 software for production of smooth maps [50]. (D) X-ray and circular dichroism-calculated secondary structures for Ca²⁺-bound S100A4 WT and mutants.