Table 1. Stemness phenotype of S100A4 WT and mutants compared in the figures.

Assay	WT	NM	CM	CD
OECM1 sphere formation	++++	+++	+	++
FaDu sphere formation	+++	++	±	+
memGRP78-OECM1f	+++	++++	++	+
memGRP78-FaDuf	++++	++	−	+++
GRP78 proteinW	+++	±	±	−
Glut3 proteinW	+++	+	+	+
Mice tumor volume	+++	−	+	++
CK18 (undifferentiation)IHC	+++	+	−	++
NanogIHC	++++	++	+++	+
p-p53 ser15 (anti-apoptosis)W	+++	+++	−	−
NanogW	++	±	−	−
Nanog reporter	+++	+	++	+
Total	38	18	4	9

Positive and negative determine as high and low expression levels in comparison with the GFP, respectively.

^fflow cytometry

^Wimmunoblotting assay

^IHCimmunochemistry