Table 2. Malignant phenotype of S100A4 WT and mutants compared in the figures.
Assay | WT | NM | CM | CD |
---|---|---|---|---|
Migration-OECM1 | +++ | − | + | ± |
Migration-FaDu | ++++ | + | ± | + |
Anchorage independent growth-OECM1 | ++++ | ++ | + | ± |
Anchorage independent growth-FaDu | ++++ | ++ | ± | + |
VimentinW | ++++ | − | − | − |
Total | 18 | 2 | 0 | 1 |
Positive and negative determine as high and low expression levels in comparison with the GFP, respectively.
immunoblotting assay