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. 2016 Oct 27;7(48):79017–79031. doi: 10.18632/oncotarget.12984

Figure 2. Expression of ING4 in CRC cells negatively regulated tube formation in vitro and inhibited blood vessel formation in vivo.

Figure 2

(A–B) Western blot was used to test the ING4 expression in the p53+/+HCT116 and HCT15 cells with ING4 overexpression (HA-ING4), ING4 knockdown (mi-ING4) and respective vector control (HA-Vec and mi-Vec). (C) ING4 in CRC cells negatively regulated tube formation. (D–E) Numbers of complete tubular structures formed by HUVECs were counted for ING4 overexpressed, knocked down and control groups (n = 3/group) in CRC cells. (F) Photographs of matrigel plugs with ING4 overexpressed or control p53+/+HCT116 and HCT15 cells excised from mice after 10 days of growth in vivo. Data are presented as means ± standard deviations. *P < 0.05, **P < 0.001 (Student's t-test).