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. 2016 Oct 12;7(48):79032–79046. doi: 10.18632/oncotarget.12593

Figure 1. miR-218-5p is elevated in bone metastases.

Figure 1

A. H&E staining (upper panel) and immunohistochemical analysis of the proliferation marker Ki-67 (lower panel) in bone metastases from breast cancer patients. Scale bar indicates 50 μm. B. Expression of miR-218-5p was determined in healthy human bone (white bars), primary breast tumors (light grey bars) and bone metastasis biopsies obtained from breast cancer patients (dark grey bars) by qRT-PCR. C. miR-218-5p expression was analyzed in non-malignant epithelial MCF-10A cells, non-metastatic MCF-7 breast cancer cells and in two sub clones of metastatic MDA-MB-231 breast cancer cells by qRT-PCR. N= 4 independent experiments. Mean values ± SEM, * p<0.05, *** p<0.001 vs. MCF-10A. D. Cell proliferation was determined in MDA-MB-231 cells after transfection with miR-218-5p, antimiR-218-5p, or non-targeting control (miR-Ctrl) using an MTS Assay. N= 4independent experiments. Mean values ± SEM, * p<0.05 vs. miR-Ctrl.