Skip to main content
. 2016 Nov 1;7(48):79187–79202. doi: 10.18632/oncotarget.13007

Figure 2. Continuous in vivo interleukin re-stimulation is not necessary for NK cells to produce increased amount of IFNγ.

Figure 2

Rag1KO B6 recipient mice were divided into two groups: the continuous IL re-stimulation group and the IL re-stimulation only once group. Both groups of mice received the same doses of IL-12 (4.5 μg/mouse), IL-15 (5.5 μg/mouse), and IL-18 (22 ng/mouse) for pre-activation. After three weeks, mice further received IL-12 (5 μg/mouse) and IL-15 (8 μg/mouse) for re-stimulation either only once (the IL re-stimulation only once group) or weekly (the continuous IL re-stimulation group). The percentages of NK cells proliferation (A) and IFNγ+ NK cells (B) were determined by flow cytometry every week after IL re-stimulation. (□) the IL re-stimulation only once group; (■) the continuous IL re-stimulation group. Values indicate mean ± SEM (n = 12 per group, *p < 0.05, **p > 0.01, and ns = not significant, p > 0.05).