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. 2016 Nov 1;7(48):79187–79202. doi: 10.18632/oncotarget.13007

Figure 6. IFNγ production plays an important role in anti-leukemia activity of NK cells induced by in vivo IL pre-activation and re-stimulation.

Figure 6

(A) Rag1KO B6 (CD45.2+) mice were divided into two groups: one group of mice received IgG1 as control (IL, ■), and another group of mice received anti-IFNγ antibody for neutralization (IL+INF-g mAb, □). Both groups of mice received pre-activation (4.5 μg/mouse IL-12, 5.5 μg/mouse IL-15, and 22 ng/mouse IL-18) and re-stimulation (5 μg/mouse IL-12 and 8 μg/mouse IL-15) prior to treatment with the antibodies and 1 × 104 Notch1-T-ALL cells post IFNγ neutralization. Kaplan-Meier analysis was performed to assess survival of mice (median survival time: 75 days for the IgG1 control group and 48 days for the anti-IFNγ mAb group. Values indicate mean ± SD (n = 10 per group, p < 0. 05). (B) In parallel, mRNA expression levels of NKG2D were determined by qRT-PCR (n = 10 per group, ns = not significant, p > 0.05).