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. 2016 Nov 1;7(48):79203–79216. doi: 10.18632/oncotarget.13008

Figure 1. In absence of ligand stimulation, EHD3 increases the level of EGFR in glioblastoma cells.

Figure 1

(A) Protein extracts from U251tetEhd3 cells induced (+) or not (−) with Dox, for 1– 3 days, were examined by immunoblotting for EGFR expression. (B) The same analysis was also performed on U87tetEhd3 cells. Histograms depict densitometric quantitation of 3 different blots each and the results are shown as relative units (r.u.) in comparison to the control without Dox being the 100% value. (C) Real-time RT-PCR analysis of control (−) or Dox-induced (+) U251tetEhd3 and U87tetEhd3 cells shows that EHD3 expression does not affect EGFR expression at the transcript level. Results are shown as fold change in expression in Dox-induced versus the control without Dox treatment.