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. 2016 Nov 1;7(48):79203–79216. doi: 10.18632/oncotarget.13008

Figure 3. Upon EGF ligand stimulation, EGFR signal attenuation is more pronounced in EHD3-expressing cells.

Figure 3

(A) U251tetEHD3 cells were induced (+Dox) or not (−Dox) to express EHD3 by Dox treatment, serum starvation-primed overnight prior to stimulation with EGF at different time points. Whole cell lysates were analyzed by immunoblotting for the levels of total EGFR, tyrosine phosphorylated EGFR (pEGFR), EHD3 and GAPDH. (B) Densitometric quantitation of the signal intensities were quantified using the ImageJ software (NIH) from three different immunoblots and presented as relative units (r.u.). (C) The kinetic of EGFR activation and signal attenuation was similar in U87tetEhd3 cells. (D) Similarly, the kinetic of EGFR phosphorylation was analyzed by densitometry measurements and presented as relative units (r.u.). Statistical significance was calculated with a two-tailed t test using GraphPad Prizm 5.0. ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001.