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. 2016 Nov 1;7(48):79203–79216. doi: 10.18632/oncotarget.13008

Figure 4. EHD3 expression inactivates EGFR in vivo.

Figure 4

(A) U251tetEhd3 cells were grown as xenografts injected sub-cutaneously to nude mice given (+) or not (−) Dox in drinking water at 2 mg/ml [8]. Tumors were retrieved from 4 mice each group and whole cell lysates were analyzed by immunoblotting for the levels of total EGFR, tyrosine phosphorylated EGFR (pEGFR), EHD3 and GAPDH. (B) Signal intensities were quantified using the Image Studio Lite software (LI-COR Biosciences) and the indicated ratios presented as relative units (r.u.). Statistical significance was calculated with a two-tailed t test using GraphPad Prizm 5.0. *p ≤ 0.05; ***p ≤ 0.001.