Figure 6. The pattern of subcellular trafficking of EGFR upon EGF ligand stimulation is regulated by EHD3.

U251tetEhd3 cells were plated on glass coverslips in presence (Dox) or not (Cont) of Dox. They were incubated with an anti-EGFR antibody (Genentech) prior to stimulation with EGF for the indicated time points. Cells were then fixed, permeabilized, and stained with an alexafluor-594-conjugated secondary antibody, along with DAPI for nucleus visualization. The cells were visualized with a Leica TCS SP5 Laser (Point) Scanning Confocal microscope. Red arrowheads in control samples at 30 min indicate EGFR accumulation in the perinuclear recycling compartment, which is quasi-absent from the Dox-induced counterpart.