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. 2016 Nov 2;7(48):79247–79261. doi: 10.18632/oncotarget.13018

Figure 10. Bcl-2 immunohistochemistry and Western blots.

Figure 10

Representative examples of microphotographs from 24 hours after hypoxic-ischemia in the hippocampal CA3 (A) and cortex (C). (B) Western blot analysis of Bcl-2 levels in the hippocampus. β-Actin served as a protein loading control. The protein Bcl-2 contents were significantly higher in hippocampus of the RSV animals 24 hours after hypoxic-ischemia than the HI animals (upper panel B). Representative Western blot detected with anti-Bcl-2 antibody (lower panel B). Scale = 50 μm. Values are mean ± SEM, n = 5 rats per group. RSV vs. HI, *p < 0.05; Sham vs. HI, #p < 0.05.