Figure 1. Generation and characterization of BM-derived MSC-DF.

(A) MSC-DF are αSMA⁺/vimentin⁺/FSP1⁺ by IF. (B) Effect of Notch1 activation on MSC-DF cell growth and migration. top: expression of mutant N1^IC and elevated levels of Hes1 and Hey1 protein in Cre/Lenti-transduced MSC-DF^N1IC+/+. Decreased cell growth (middle) and migration (bottom) rates of MSC-DF^N1IC+/+ vs. MSC-DF^LSL-N1IC (non-transduced (−) or GFP/Lenti-transduced). (C) Effect of Notch1 deletion on MSC-DF cell growth and migration. top: Notch1 deletion at protein levels; middle: comparable growth rate of MSC-DF^{Notch1−/−} vs. MSC-DF^Notch1+/+; bottom: MSC-DF^{Notch1−/−} migrate faster than MSC-DF^Notch1+/+ (LPF: low power field).