Figure 5. Expression of WISP1 depends upon Notch1.

(A) Heatmap of differentially expressed gene profiles from duplicates of MSC-DF^{Notch1−/−} vs. MSC-DF^Notch1+/+ by microarray analysis. (B) Heatmap of differentially expressed CCN family genes (top). Relative levels of CCN1-5 genes in MSC-DF^{Notch1−/−} vs. MSC-DF^Notch1+/+ (bottom), based on unpaired Student's t-test between the two conditions. (C) Deletion of Notch1 in MSC-DF^{Notch1−/−} (top) or expression of N1^IC in MSC-DF^N1IC+/+ (bottom) and downregulation of WISP1 in MSC-DF^{Notch1−/−} are validated by immunoblotting.