Skip to main content
. 2016 Oct 15;7(48):79388–79400. doi: 10.18632/oncotarget.12692

Figure 4. Rigosertib induces ROS generation and activates JNK pathway signaling in HNSCC cell lines.

Figure 4

A. Histogram plot of ROS levels as measured by flow cytometry. FaDu cells were incubated with 10.0 μM DCF-DA for 30 min, washed, then incubated with DMSO (Ctrl), 1.0 μM ON 01911.Na (control compound), or 0.1 to 10.0 μM rigosertib for 6 h before assessing ROS levels by flow cytometry. Data represent of 3 independent experiments. B. Representative Western blot analysis of the effects of rigosertib on JNK pathway signaling proteins. FaDu cells were incubated with DMSO (Ctrl), or increasing concentrations of ON 01911.Na (control compound) or rigosertib for 24 h before protein evaluation. GAPDH used as loading control. C. Representative Western blot analysis of the effects of rigosertib on the phosphorylation of JNK and ATF2 and total ATF2 in multiple HNSCC cell lines. UMSCC 1, UMSCC 47 and UMSCC 104 cells were incubated with 1.0 μM ON 01911.Na (control compound) or 1.0 μM rigosertib for 24 h before protein evaluation. GAPDH used as loading control.