Skip to main content
. 2016 Oct 19;7(48):79526–79543. doi: 10.18632/oncotarget.12755

Figure 3. The effect of GDC-0941 treatment on viability in a panel of NSCLC cells.

Figure 3

H460, A549, and H1975 cells were treated at noted concentrations of GDC-0941 for 48 hours in a 96 well plate and then fixed & stained with Höechst nuclear stain, mitotracker red mitochondrial stain and phalloidin green F-actin stain. A. Stained plates were imaged using the InCell 1000 (10X objective, 10 fields per well, triplicate wells, n=3). B. Images were analysed for cell count using InCell 1000 software. Cell numbers from each treatment group were compared with untreated cell numbers and are represented here as % cell count compared to untreated cells, with mean ± SEM for treated groups. */***: p<0.05/0.001 respectively, compared to untreated cells of the same cell line.