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| Primer name | Primer sequence (5′-3′) |
|---|---|
| let-7a RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACaactat |
| U6 RT | F: AAAATATGGAACGCTTCACGAATTTG |
| Let-7a Real-time PCR | F: TGAGGTAGTAGGTTG R: GTCGTATCCAGTGCAGGGTCCGAGGT |
| U6 Real-time PCR | F: CTCGCTTCGGCAGCACATATACT R: ACGCTTCACGAATTTGCGTGTC |
| Lin28A ORF | F: ggatccATGGGCTCCGTGTCCAACCAG R: acgcgtTTCTGTGCCTCCGGGAGCAG |
| Lin28B ORF | F: ggatccTTGATGGCCGAAGGCGGGGCTA R: acgcgtTTCCTTTTTTGAACTGAAGGCCCC |
