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. 2016 Oct 25;7(48):79617–79628. doi: 10.18632/oncotarget.12873

Figure 3. The expression level of miR-31 was significantly lower in CAFs than in NFs and Up- or down-regulation of miR-31 had no effect on the CAF phenotype.

Figure 3

A. The expression of miR-31 in CAF was significantly lower than in NF (*P < 0.05). B. transfection efficiency of CAF were transfected with FAM-siRNA, GFP-green fluorescence was detected with a fluorescence microscopy after 48 hours. C. No significant difference was observed in the expression of the marker protein α-SMA after the up- or down-regulation of miR-31. D. No significant difference was observed in the expression of the marker protein Vimentin after the up- or down-regulation of miR-31. miR-31 had no effect on the CAF phenotype. E. No significant difference was observed in the proliferation of CAF after the up- or down-regulation of miR-31. F. No significant difference was observed in the apoptosis of CAF after the up- or down-regulation of miR-31.