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. 2016 Oct 27;7(48):79688–79705. doi: 10.18632/oncotarget.12939

Figure 2. The co-expression of BRGs in breast cancer cells is derived from EMT that has undergone BMP2 induction.

Figure 2

MCF-7 and T47D cells were treated as indicated. A. The protein expression of SMAD1, p-SMAD1, SMAD3 and p-SMAD3, as well as EMT markers in the cancer cells was detected by immunoblot. β-actin was used as a loading control. B. and C. The protein expression of BRGs in cancer cells was detected by immunoblot. β-actin was used as a loading control. D. The protein expression and localization of FN1, VIM, E-Cad, and CDH11 (green) in MCF-7 cells was detected by immunofluorescence. DAPI staining (blue) indicates the nucleus. Scale bar, 40 μm.