Skip to main content
. 2016 Oct 27;7(48):79688–79705. doi: 10.18632/oncotarget.12939

Figure 5. RUNX2 is critical for the CAF CM/BMP2-induced advantages of homing, residing and growing of breast cancer cells in an osteblast-mimc bone microenvironment.

Figure 5

MCF-7, T47D and MDA-MB-231 cells were treated as indicated. A. The morphology of cancer cells. B. The chemotactic migration of cancer cells towards MC3T3E1 cells was assessed by transwell assay. C. The adhesion of cancer cells (red, labeled with Cy3) to MC3T3E1 cells was assessed by putting cancer cells on top of MC3T3E1 cells at 100% confluence and incubating the co-culture for 30 min. D. The colony formation of cancer cells in soft agar with MC3T3E1 CM. Magnification: 200X. E. The proliferation of cancer cells in MC3T3E1 CM. Three independent experiments were performed in duplicate. The data are presented as the mean ± SD. *, P < 0.05 compared with the corresponding siControl.