Figure 5. GPC5 inhibits lung adenocarcinoma and Epithelial-Mesenchymal Transition (EMT) in NOD-SCID mice model.

A. LAC metastatic model in vivo. 4.0×10⁶ A549-NC-L.V-luc and A549-GPC5-L.V-luc cells were injected by tail vein into SCID mice imaged for bioluminescence and analyzed by the number of photons. B. the change of average photon intensities was demonstrated by the line graph. C. representative image of the visible metastatic nodules in the mouse lungs of the A549-NC-LV-luc group and the A549-GPC5-LV-luc group (n=5, top). Average weight of the mouse lungs in the subject and the control groups were presented as the mean ± SD (bottom). D. representative HE and immunofluorescence staining images of the visible metastatic nodules from the mouse liver and lung sections of the A549-NC group and the A549-GPC5 group.