Table 1. qRT-PCR primers.
Gene symbol | Accession number | Primer | Primer sequence (5′-3′) | Product size | Tm (°C) |
---|---|---|---|---|---|
cyt-c | NM001079478 | Forward Reverse |
TGTCCAGAAATGTTCCCAGTGC CCTTTGTTCTTATTGGCATCTGTG |
138bp | 60 |
XIAP | NM204588 | Forward Reverse |
CTAAACAACGAACAGCATCCAAGG ACAACGTGATCGCCATTACCTG |
146bp | 58 |
HtrA2 | XM423666 | Forward Reverse |
CATCCAGACAGACGCCGCTAT CAGGAACTTTCGCAGTCGGTC |
145bp | 62 |
Smac | XM415152 | Forward Reverse |
TCCCAGAAGGCAGAGACCAAG GGTCCTCACCCGCATCTGTA |
118bp | 62 |
apaf1 | XM416167 | Forward Reverse |
AAGGGCATAAGGAAGCAATCAA CAGCACAAGAAAGAACAGCACC |
156bp | 61 |
caspase-9 | AY057940 | Forward Reverse |
CGAAGGAGCAAGCACGACAG CCGCAGCCCTCATCTAGCAT |
130bp | 61 |
caspase-3 | NM204725 | Forward Reverse |
TGGCCCTCTTGAACTGAAAG TCCACTGTCTGCTTCAATACC |
139bp | 62 |
caspase-6 | AF469049 | Forward Reverse |
TCAGAGGAGACAAGTGCCAGAGT TACTGAATCCTGAACGAGAACTGG |
107bp | 59 |
caspase-7 | XM421764 | Forward Reverse |
CCGAAGTCCTCACTCAGTAACCA TTGCGTGTACCCATTCCTGTT |
137bp | 58 |
Fas | NM001199487 | Forward Reverse |
TGTTCGTCATCACCGTCTATCG TTCGTAGGCTCCTCCCATCC |
133bp | 60 |
FasL | AJ890143 | Forward Reverse |
AGATCGCATCCCTCCAGCTC GAGACAGGTTCCCACTCCAATG |
135bp | 59 |
caspase-8 | NM204592 | Forward Reverse |
TGGGAAAGTGGACAAGAGCCT CCACAGATGATGCCAGCCAA |
146bp | 59 |
caspase-10 | XM421936 | Forward Reverse |
GCAGCGTTCAGAAGACCACAA CATTGCTTGGCAGTGAAGTAGGT |
141bp | 61 |
Bid | NM204522 | Forward Reverse |
AGTGGAAGGACTTGCCAGAGC TTGTGGAAGTGTTGGCTGATGTA |
162bp | 60 |
PARP | NM205263 | Forward Reverse |
AAGCTCCGAACTGATATTAAGGTGG GCTTAAATGGCTTGTAACGCTGA |
172bp | 56 |
β-actin | L08165 | Forward Reverse |
TGCTGTGTTCCCATCTATCG TTGGTGACAATACCGTGTTCA |
178bp | 62 |