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. 2016 Nov 4;7(48):79869–79884. doi: 10.18632/oncotarget.13084

Figure 2. Individual RSK isoforms regulate GBM cell invasion in 3D.

Figure 2

A. Stable U-373 MG cell lines with knocked down RSK1, -2, -3, or -4 isoform expression (shRSK1-4) or cells carrying a scrambled control vector (scr) were generated using two independent shRNA constructs targeting RSK1-4. RSK1-4 knock-down and control cell lines were subjected to a tumor spheroid invasion assay. Spheroids were embedded in a 50% matrigel-50% collagen I matrix and invasion was analyzed after 48 hours. Quantification at 48 hours is shown. B. RSK1-4 isoform knock-down had no effect on GBM cell viability at 48 hours. C. Protein knock-down levels were determined by immunoblotting as indicated.