Skip to main content
. 2016 Nov 4;7(48):79869–79884. doi: 10.18632/oncotarget.13084

Figure 5. Patient-derived GBM cells require RSK2 for invasion and migration.

Figure 5

A. RSK1-4 are expressed in patient cells and are phosphorylated at S386 and T573 indicating likely activation. B. Quantification of RSKs normalized to tubulin with cumulative expression in patients indicated for each. C. Quantification of scratch assay migration experiments and D. transwell assay. At 24 hours, RSK2 gene knock-down cells and wild-type cells treated with BI-D1870 significantly decreased cell migration in both scratch (t-test; P = 0.0036 and 0.00043, respectively) and transwell assays (t-test; P = 0.0061 and 0.011, respectively). Images of example scratch assays are shown (C). Scratch assays were quantified using ImageJ and normalized to time 0 h. E. There was no effect on cell number or morphology in the migration assays.