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. 2016 Nov 7;7(48):79925–79942. doi: 10.18632/oncotarget.13160

Figure 6. Rac1 inhibition reverts CD99-mediated effects.

Figure 6

A. AO staining was performed in 6647 cells before (CTR) or after exposure with 0662mAb (3h) alone or combined with the Rac1 inhibitor EHT 1864 (25 μM). For AO staining, cells were acquired using the microscope Nikon ECLIPSE 90i with Plan Fluor 40x/0.75 DIC M/N2. Pictures provided are all merged images (scale bar 20μm). B. Percentage of 6647 live cells before (−) and after (+) treatment with anti-CD99 0662mAb alone, or with Rac1 inhibitor EHT 1864 (25 μM for 48 h pretreatment) (trypan blue dye exclusion). C. Percentage of live 6647 cells before (−) and after (+) treatment with anti-CD99 0662mAb and/or with siRNA against Rac1. Cells were transfected by a scrambled (SCR) as control (trypan blue dye exclusion). Results are expressed as mean ± SEM of three or more independent experiments (Student's t test: *p< 0.05; **p<0.01).