Table 5. Primer and probe sequences used for the PD-L1 methylation-specific qPCRs.
Primer/Probe | Sequence |
---|---|
PD-L1 forward primer | 5′-ATATAAAATAAATAATCATTCTTATACG-3′ |
PD-L1 reverse primer | 5′-CGTTTAGGGATTTTGGATTTGTTTAGC-3′ |
PD-L1 detection probe | 5′-FAM-CACGAATCCAAATCCACCGCCAAC-BHQ1-3′ |
ACTB forward primer | 5′-GTGATGGAGGAGGTTTAGTAAGTT-3′ |
ACTB reverse primer | 5′-CCAATAAAACCTACTCCTCCCTTAA-3′ |
ACTB detection probe | 5′-Atto 647N-ACCACCACCCAACACACAATAACAAACACA-BHQ1-3′ |