Table 5. Primer and probe sequences used for the PD-L1 methylation-specific qPCRs.

Primer/Probe	Sequence
PD-L1 forward primer	5′-ATATAAAATAAATAATCATTCTTATACG-3′
PD-L1 reverse primer	5′-CGTTTAGGGATTTTGGATTTGTTTAGC-3′
PD-L1 detection probe	5′-FAM-CACGAATCCAAATCCACCGCCAAC-BHQ1-3′
ACTB forward primer	5′-GTGATGGAGGAGGTTTAGTAAGTT-3′
ACTB reverse primer	5′-CCAATAAAACCTACTCCTCCCTTAA-3′
ACTB detection probe	5′-Atto 647N-ACCACCACCCAACACACAATAACAAACACA-BHQ1-3′