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HULC knockdown suppressed GC cells apoptosis.
(A) The efficiency of HULC knockdown was confirmed via qRT-PCR in two independent human gastric cancer cell lines, MGC-803 and MKN-45. (B) Flow cytometry detected the apoptosis rate using double-staining with Annexin V and PI in MGC-803 and MKN-45 cells exposed to CDDP.
Each experiment was repeated at least three times using independent biological replicates. *, p<0.05 compared with control.
