Figure. 2.

Gel electrophoresis and Melting curve analysis. (a) Gel electrophoresis of the PCR products. M: Molecular Size marker -100bp ladder. Lane1: 102 bp PCR product of GAPDH gene. Lane 2: Non Template Control for GAPDH gene. Lane 3: 204 bp PCR product of NM23. Lane 4: Non Template Control for NM23 gene. (b) The melting curve at 86.5  C for GAPDH gene and 85.2  C for NM23 gene indicated the specific products that melt at the different temperature. Flat peak demonstrates Non Template Control: NTC