Table 1.

Primers and protocols used for the amplification of Cytauxzoon sp., Hepatozoon spp. and housekeeping GAPDH gene control

	PCR primers (5′-3′)	PCR conditions	Product size (bp)
PCR-1	F: CCAGCAGCCGCGGTAATTC	94 °C, 3 min; 35 cycles [94 °C, 30 s, 64 °C, 45 s, 72 °C, 30 s]; 72 °C, 7 min	373
	R: CTTTCGCAGTAGTTYGTCTTTAACAAATCT
PCR-2	F: CCTGGTTGATCCTGCCAG	96 °C, 3 min; 40 cycles [96 °C, 1 min, 65 °C, 1 min, 72 °C, 2 min]; 72 °C, 1 min	1,675
	R: CGACTTCTCCTTCCTTTAAG
Housekeeping (GAPDH)	F: CCTTCATTGACCTCAACTACAT	95 °C, 1 min; 45 cycles [94 °C, 4 s, 57 °C, 4 s, 72 °C, 3 s]; 72 °C, 1 min	282
	R: CCAAAGTTGTCATGGATGACC