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. 2017 Mar 13;10:113. doi: 10.1186/s13071-017-2050-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Scatter plot showing reactivity of sera tested by ELISA from eight dogs infected with Leishmania infantum. Seroreactivity was tested with antigens of L. infantum, L. major and L. tropica. Y-axis shows calibrated optical density (OD). The thick black line represents the mean value. The intermittent line represents the serological cut-off value. No significant differences were found between OD readings of the same sera with the different antigens (F _(2,23) = 0.72, P = 0.498)