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. 2017 Mar 13;10:113. doi: 10.1186/s13071-017-2050-7

Fig. 5.

Fig. 5

Scatter plot showing reactivity of six sera tested by ELISA from two dogs infected with Leishmania major. Seroreactivity was tested with antigens of L. infantum, L. major and L. tropica. Y-axis shows calibrated optical density (OD). The thick black line represents the mean value. The intermittent line represents the serological cut-off value. Samples 6835 and 0026 were omitted from the mean calculation due to dog case no. 2 being seronegative. No significant differences were found between OD readings of the same sera with the different antigens (F (2,11) = 0.118, P = 0.824)