Table 1.
Frequency of microscopic lesions and detection of Leishmania by means of HE, IHC and qPCR on paired skin samples from the dogs studied based on disease stage. Values with the same superscript differ significantly
| Skin samples | Microscopic lesions | Detection of Leishmania | ||
|---|---|---|---|---|
| HE | IHQ | qPCR | ||
| Normal-looking skin (n = 25) | 14/25 (56.0 %) | 5/25 (20%) | 8/25 (32.0%) | 18/25 (72.0%) |
| Stage I (n = 11) | 3/11 (27.3%)a,b | 0/11 (0%)c | 1/11 (9.1%)d | 5/11 (45.5%)e |
| Stage II-III (n = 14) | 11/14 (78.6%)b | 5/14 (35.7%)c | 7/14 (50.0%)d | 13/14 (92.9%)e |
| Clinically-lesioned skin (n = 25) | 25/25 (100%) | 11/25 (44.0%) | 23/25 (92.0%) | 25/25 (100%) |
| Stage I (n = 11) | 11/11 (100%)a | 1/11 (9.1%)f | 9/11 (81.8%) | 11/11 (100%) |
| Stage II-III (n = 14) | 14/14 (100%) | 10/14 (71.4%)f | 14/14 (100%) | 14/14 (100%) |
Abbreviations: HE haematoxylin and eosin stained sections, IHC Leishmania immunohistochemistry, qPCR quantitative PCR
aMcNemar's test: P = 0.008
bFisher’s exact test: P = 0.0172
cFisher’s exact test: P = 0.0464
dFisher’s exact test: P = 0.0421
eFisher’s exact test: P = 0.0068
fFisher’s exact test: P = 0.0037