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. 2017 Mar 13;10:130. doi: 10.1186/s13071-017-2063-2

Table 1.

Polymerase chain reaction details for the qPCR/PCR assays used in the study for the testing of infectious agents

Target species (target gene) PCR primer or probe sequences (5'–3') Product size (bp) Reference
Bartonella henselae (alr-gcvP intergenic spacer) F: GAGGGAAATGACTCTCTCAGTAAAA 110 [20]a
R: TGAACAGGATGTGGAAGAAGG
FAM-CAGCCAAATATACGGGCTATCCATCAA-TAMRA
“Candidatus Mycoplasma haemominutum” (16S rRNA gene) F: TGATCTATTGTKAAAGGCACTTGCT 135 [18]
R: TTAGCCTCYGGTGTTCCTCAA
FAM-TTCAATGTGTAGCGGTGGAATGCGT-BHQ1
“Candidatus Mycoplasma turicensis” (16S rRNA gene) F: AGAGGCGAAGGCGAAAACT 138 [18]
R: ACGTAAGCTACAACGCCGAAA
FAM-CGTAAACGATGGGTATTAGATGTCGGGAT-BHQ1
Ehrlichia/Anaplasma spp. (16S rRNA gene) F: GGTACCYACAGAAGAAGTCC 345 [21]
R: TAGCACTCATCGTTTACAGC
Hepatozoon. spp. (18S rRNA gene) F: AAACGGCTACCACATNTAAGGA 522
R: AATACAAATGCCCCCAACTNT
Leishmania spp. (screening assay) (kinetoplast DNA, kDNA) F: CGGGTAGGGGCGTTCTG 115 [19]
R: ATTTTACACCAACCCCCAGTT
FAM-TGGGTGCAGAAATCCCGTTCA-BHQ1
Leishmania spp. (confirmatory assay) (kDNA) F: CCTATTTTACACCAACCCCCAGT 120 [22, 23]
R: GGGTAGGGGCGTTCTGCGAAA
Mycoplasma haemofelis (16S rRNA gene) F: GTGCTACAATGGCGAACACA 80 [18]
R: TCCTATCCGAACTGAGACGAA
FAM-TGTGTTGCAAACCAGCGATGGT-BHQ1

Abbreviations: F forward primer sequence, R reverse primer sequence, FAM 6-carboxyfluorescein on the Taqman probe, BHQ1 black hole quencher 1 on the Taqman probe, TAMRA Carboxytetramethylrhodamine on the Taqman probe

aThe reverse and probe sequences in the original paper are incorrectly labelled; the correct sequences are cited in this table