Table 1.
Polymerase chain reaction details for the qPCR/PCR assays used in the study for the testing of infectious agents
| Target species (target gene) | PCR primer or probe sequences (5'–3') | Product size (bp) | Reference |
|---|---|---|---|
| Bartonella henselae (alr-gcvP intergenic spacer) | F: GAGGGAAATGACTCTCTCAGTAAAA | 110 | [20]a |
| R: TGAACAGGATGTGGAAGAAGG | |||
| FAM-CAGCCAAATATACGGGCTATCCATCAA-TAMRA | |||
| “Candidatus Mycoplasma haemominutum” (16S rRNA gene) | F: TGATCTATTGTKAAAGGCACTTGCT | 135 | [18] |
| R: TTAGCCTCYGGTGTTCCTCAA | |||
| FAM-TTCAATGTGTAGCGGTGGAATGCGT-BHQ1 | |||
| “Candidatus Mycoplasma turicensis” (16S rRNA gene) | F: AGAGGCGAAGGCGAAAACT | 138 | [18] |
| R: ACGTAAGCTACAACGCCGAAA | |||
| FAM-CGTAAACGATGGGTATTAGATGTCGGGAT-BHQ1 | |||
| Ehrlichia/Anaplasma spp. (16S rRNA gene) | F: GGTACCYACAGAAGAAGTCC | 345 | [21] |
| R: TAGCACTCATCGTTTACAGC | |||
| Hepatozoon. spp. (18S rRNA gene) | F: AAACGGCTACCACATNTAAGGA | 522 | |
| R: AATACAAATGCCCCCAACTNT | |||
| Leishmania spp. (screening assay) (kinetoplast DNA, kDNA) | F: CGGGTAGGGGCGTTCTG | 115 | [19] |
| R: ATTTTACACCAACCCCCAGTT | |||
| FAM-TGGGTGCAGAAATCCCGTTCA-BHQ1 | |||
| Leishmania spp. (confirmatory assay) (kDNA) | F: CCTATTTTACACCAACCCCCAGT | 120 | [22, 23] |
| R: GGGTAGGGGCGTTCTGCGAAA | |||
| Mycoplasma haemofelis (16S rRNA gene) | F: GTGCTACAATGGCGAACACA | 80 | [18] |
| R: TCCTATCCGAACTGAGACGAA | |||
| FAM-TGTGTTGCAAACCAGCGATGGT-BHQ1 |
Abbreviations: F forward primer sequence, R reverse primer sequence, FAM 6-carboxyfluorescein on the Taqman probe, BHQ1 black hole quencher 1 on the Taqman probe, TAMRA Carboxytetramethylrhodamine on the Taqman probe
aThe reverse and probe sequences in the original paper are incorrectly labelled; the correct sequences are cited in this table